[Adjuvant effect of the Cry1Ac protoxin on cellular immunity when is coadministered with a good mucosal pathogen]

The major part of pathogens use the mucosae to penetrate into the organisms, the idea to blockade the invasion, replication, colonization and multiplication of them results attractive not only to vaccines design, but immunopharmacs design, too. The protoxin Cry1Ac from Bacillus thuringiensis is a recent known mucosal adjuvant.

To determine the adjuvant effect of protoxin CrylAc from Bacillus thuringiensis on cellular response.

Mononuclear cells from human peripheral blood were isolated in Ficoll-Hypaque gradients (d=1077), those cells CD3-CD14+ were obtained with the help of FACSVantage system, and then were stimulated with a lisate of S. pyogenes alone or combined with different concentrations of CrylAc. The proliferation of monocytes was determined in experiments of timidina-3H incorporation.

The proliferative response of monocytes was major in those stimulated with S. pyogenes lisate and CrylAc than the observed when monocytes were stimulated with lisate alone. The major response was obtained with lisates of S. pyogenes plus 12.5 microg/mL of CrylAc (p<0.05).

CrylAc have an important adjuvant effect on the cellular immune response. These findings are important to treat infections and for vaccines design.