Synthesis and properties of 2'-O-cyanoethylated RNA derivatives
Hisao Saneyoshi1, Keigo Tamaki, Kohji Seio
1Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
Nucleic Acids Symposium Series (2004)
|December 8, 2006
Abstract:
We developed a new protocol for the cyanoethylation of the hydroxyl function of nucleosides. A new system of Cs2CO3-tBuOH proved to be the best for the cyanoethylation. An oligonucleotide incorporating 2'-O-cyanoethylated uridine has high affinity (deltaTm = 0.6 degrees C/modification) for the complementary DNA or RNA strand than the corresponding 2'-O-methyl-RNA.
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