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Updated: Jul 18, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
Site-specific fluorescent labeling of RNA by a base-pair expanded transcription system
Rie Kawai1, Michiko Kimoto, Tsuneo Mitsui
1Protein Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Abstract:
Ribonucleoside 5'-triphosphates of fluorescently-labeled 2-oxo(1H)pyridines, in which carboxyfluorescein (FAM) or carboxytetramethylrhodamine (TAMRA) was attached at position 5, were chemically synthesized. These substrates were site-specifically incorporated into RNA by T7 transcription mediated by unnatural base pairs between 2-amino-6-(2-thienyl)purine and 2-oxo(1H)pyridine or 2-amino-6-(2-thiazolyl)purine and 2-oxo(1H)pyridine. This site-specific fluorescent labeling of RNA fragments will be a powerful tool for the fields of nucleic acid chemistry, biology, and technology.
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