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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
2',4'-BNA derivatives bearing an unnatural nucleobase: synthesis and application to triplex-forming oligonucleotides
Hiroyasu Inohara1, Satoshi Obika, Takeshi Imanishi
1Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract:
Recognition of dsDNA by a triplex-forming oligonucleotide (TFO) is limited to homopurine x homopyrimidine sequences. Therefore, it is necessary to develop novel nucleoside analogues which recognize pyrimidine x purine basepairs (C x G or T x A). We have designed and synthesized novel 2',4'-BNA/LNA monomers bearing 3-hydroxybenzene and indole as a nucleobase (3HB(B) and In(B)), and these nucleoside analogues have been introduced into TFOs. On melting temperature (Tm) measurements, 3HB(B) and In(B) were found to interact with T x A base pair interruption with moderate binding affinity.
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