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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Base-discriminating fluorescent DNA probe based on the guanine-specific fluorescence quenching
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8510, Japan.
Abstract:
A new strategy for the detection of a single base alteration utilizing the fluorescence quenching by guanine base is described. We have devised a novel base-discrimination fluorescent (BDF) nucleoside, PyT, which contains pyrene carboxamide fluorophore at sugar 4' position. PyT-containing oligodeoxynucleotides (ODNs) exhibit an intense fluorescence only when PyT forms a complementary base pair with adenine.
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