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Updated: Jul 18, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
Published on: March 17, 2023
Assembling liposomes by means of an oligonucleotide tagged with a lipophilic unit
Naoto Maru1, Ken-ichiroh Shohda, Tadashi Sugawara
1Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Abstract:
We synthesized DNA-cholesterol conjugate molecules and introduced them into 1-palmitoyl-2-oleoyl-sn-3-phosphocholine (POPC) liposomes to create DNA-tagged liposomes. These liposomes aggregated selectively depending on the sequence of DNA tag and the resultant pattern of the aggregation was a wide-spreading network structure, which was observed under a phase contrast microscope. The structure was minutely analyzed by transmission electron microscopy (TEM) and confocal laser-scanning microscopy. As a result, a hierarchical structure of the amphiphilic molecules was constructed from the liposome to the network via clustered structures.

