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Updated: Jul 18, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
Published on: June 19, 2018
Spectroscopic analysis of DNA duplexes including T-T mismatches
Hiroshi Yamaguchi1, Shuji Oda, Yoshinori Kondo
1Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Abstract:
The DNA duplex with tandem T x T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches, TT10:d(CGCGTTGTCC) x d(GGACTTCGCG). From two-dimensional (2D) 1H-1H NOESY spectrum, we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all the base protons and anomeric protons. These results indicate that the overall structure of the duplex is a right-handed duplex, with two successive T x T mismatches stacked in the helix.
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