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Updated: Jul 18, 2026

Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
Analysis of the secondary structure of a ribozyme that requires His as a cofactor
Junji Kawakami1, Chisako Maiya, Naoki Sugimoto
1Department of Chemistry, Faculty of Science and Engineering, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Abstract:
A novel ribozyme that requires L-histidine for expression of the function was revealed from an in vitro selection experiment. Here, we report the secondary structural analysis of the ribozyme. Several deletion and point mutants were designed based on the possible secondary structures predicted by a thermodynamic method with nearest neighbor parameters. As a result, formation of two important stems was essential for folding into the active structure.
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