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Properties of ferrocene-polyamide compounds as redox active DNA binding molecules toward the SNPs detection
Masahiro Mizuta1, Takeshi Terada, Kohji Seio
1Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.
Abstract:
Ferrocene-polyamides having not only sequence discrimination ability but also a redox mediator were synthesized. The affinity and selectivity of these compounds for a DNA duplex having a cognate GCG sequence were investigated by CD spectroscopy. Furthermore, the binding of the ferrocene-polyamides to DNA duplexes immobilized to a gold electrode was detected by cyclic voltammetry (CV) experiments. The electrochemical studies revealed that the fully matched DNA duplex having a GCG sequence showed the largest electric current, and the introduction of a mismatch site decreased the current significantly. These results suggested the potential of the ferrocene-polyamide compounds as new electrochemical ligands for electrochemical SNPs detection.
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