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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
Published on: October 27, 2011
Regulation of eukaryotic DNA replication by proliferation associated protein, PA2G4, in vitro
Shunji Izuta1, Miki Kitahara, Tomoko Hamaguchi
1Department of Biological Science, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Abstract:
To search a novel factor possibly involved in the regulation of DNA replication for eukaryotic cells, we fractionated the Xenopus egg extract frequently used as a cell free DNA replication system, and obtained a single polypeptide of 50 KDa on SDS-polyacrylamide gel electrophoresis. Data of the amino acid sequence reduced from cloned gene of this protein revealed that this might be a Xenopus homolog of human PA2G4 that was known as a proliferation-associated protein or a cell cycle-regulated protein. Recombinant mouse PA2G4 protein also showed the inhibitory effect on the DNA replication reaction in Xenopus egg extract with the single-stranded DNA template. These results strongly suggest that PA2G4 may have an important role on the regulation of DNA replication in eukaryotic cells.
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