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Two-dimensional fractionation of complex mixture of oligonucleotides in microRNA size range

Hideaki Shiraishi1

  • 1Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.

Nucleic Acids Symposium Series (2004)
|December 8, 2006
PubMed

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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