Properties and EPR studies of oligodeoxynucleotides containing 2-N-tert-butylaminoxylpurines
Mariko Aso1, Toshiyuki Kaneko, John Walter Mirc
1Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Nucleic Acids Symposium Series (2004)
|December 8, 2006
Abstract:
Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.
