Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Efforts to expand the genetic code.

Floyd E Romesberg1

  • 1The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.

Nucleic Acids Symposium Series (2004)
|December 8, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery, implications and initial use of semi-synthetic organisms with an expanded genetic alphabet/code.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2023
Same author

Creation, Optimization, and Use of Semi-Synthetic Organisms that Store and Retrieve Increased Genetic Information.

Journal of molecular biology·2021
Same author

Transcriptional processing of an unnatural base pair by eukaryotic RNA polymerase II.

Nature chemical biology·2021
Same author

Efforts toward Further Integration of an Unnatural Base Pair into the Biology of a Semisynthetic Organism.

Journal of the American Chemical Society·2021
Same author

Genetic Code Expansion: Inception, Development, Commercialization.

Journal of the American Chemical Society·2021
Same author

Transcription and Reverse Transcription of an Expanded Genetic Alphabet In Vitro and in a Semisynthetic Organism.

Journal of the American Chemical Society·2020
Same journal

A novel convenient method for high bacteriophage titer assay.

Nucleic acids symposium series (2004)·2009
Same journal

Expression behavior of high-pressure-compacted plasmid DNA in mammalian cell.

Nucleic acids symposium series (2004)·2009
Same journal

Role of exposed aromatic residues in substrate-binding of CBM family 5 chitin-binding domain of alkaline chitinase.

Nucleic acids symposium series (2004)·2009
Same journal

Incipient complex formation between AP endonucleases and DNA containing AP site: a vital role of the tryptophan residue.

Nucleic acids symposium series (2004)·2009
Same journal

Physiological role of RsgA in ribosome biosynthesis.

Nucleic acids symposium series (2004)·2009
Same journal

Trans-translation by tmRNA and SmpB.

Nucleic acids symposium series (2004)·2009
See all related articles

Researchers developed novel unnatural base pairs by modifying phenyl rings, expanding the genetic code. Some pairs show stability and efficient synthesis, with ongoing efforts to evolve DNA polymerases for better recognition.

Area of Science:

  • Synthetic biology
  • Molecular biology
  • Biochemistry

Background:

  • Expanding the genetic code is crucial for novel protein functions.
  • Unnatural base pairs (UBPs) offer a route to genetic code expansion.
  • Previous UBPs faced challenges with stability and polymerase recognition.

Purpose of the Study:

  • To design and synthesize novel UBPs with reduced aromaticity.
  • To assess the stability and synthesis efficiency of these new UBPs.
  • To develop DNA polymerases capable of efficiently utilizing these UBPs.

Main Methods:

  • Synthesized phenyl ring derivatives with methyl, fluoro, or nitrogen substituents.
  • Evaluated UBP stability and polymerase-mediated synthesis efficiency.
  • Employed activity-based selection systems to evolve DNA polymerases.

Related Experiment Videos

Main Results:

  • Several novel UBPs demonstrated surprising stability and reasonable synthesis efficiency.
  • Reduced aromatic surface area did not preclude UBP formation and function.
  • Initial successes were achieved in evolving DNA polymerases for UBP recognition.

Conclusions:

  • Modified phenyl ring-based UBPs are viable candidates for genetic code expansion.
  • The design strategy effectively addressed potential issues like intercalation.
  • Further evolution of DNA polymerases will enhance the utility of these UBPs.