Biophysical and biochemical properties of oxanine-containing oligodeoxynucleotide
Seung Pil Pack1, Mitsuru Nonogawa, Nagendra Kumar Kamisetty
1International Innovation Center, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
Abstract:
Since oxanine (Oxa) was found as one of the major products from nitrosative oxidation of guanine, Oxa has been expected as mutagenic lesion related to NO-induced DNA damage. Here, to elucidate the mutagenic significance of Oxa in vitro, several Oxa-containing oligodeoxynucleotides (Oxa-ODNs) were synthesized and subjected to biophysical and biochemical characterization. Oxa-ODNs were analyzed in terms of their duplex stabilities and DNA polymerase responses. These results will be helpful in understanding the genotoxic pattern induced by Oxa.
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