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Updated: Feb 19, 2026

08:49
Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
Published on: September 16, 2019
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SNPs analysis by use of oligonucleotides containing 2'-O-methyl-2-thiouridines
Itaru Okamoto1, Kohji Seio, Mitsuo Sekine
1Graduate School of Life Science, Tokyo Institute of Technology.
Nucleic Acids Symposium Series (2004)
|December 8, 2006
Abstract:
In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.

