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Updated: Jul 18, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Development of new N-unprotected phosphoramidite building blocks having a silyl-type linker
Akihiro Ohkubo1, Katsufumi Aoki, Haruhiko Taguchi
1Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
Abstract:
New silyl-type linkers 2 and 3 were developed for the synthesis of oligonucleotides having base-labile functional groups in the phosphoramidite method without base protection. These linkers could be introduced into polymer supports in high yields and easily cleaved under neutral conditions. Particularly, the use of the N-unprotected phosphoramidite building block 3 having a silyl-type linker enabled us to synthesize oligodeoxynucleotides without protecting groups for the amino groups of 3'-terminal deoxyribonucleosides.
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