Preparation of modified oligonucleotides by the post-synthetic modification on resin method

We report the novel method that accomplishes many processes such as synthesis, modification, deprotection, washing, on the solid support. Using polymers with a novel linker, oligonucleotides has been synthesized on a DNA synthesizer by the established procedures, then the polymer was treated in alkaline solutions for removing the protection groups. Since the linker is stable in alkaline solutions, oligonucleotides were still connected to the polymers. After washing out the chemical impurities, the oligonucleotides were released from the polymer by light irradiations.