Related Experiment Video
Updated: Jul 18, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Use of abasic site-containing DNA for electrochemical SNPs detection
Kotaro Morita1, Seiichi Nishizawa, Norio Teramae
1Department of Chemistry, Graduate School of Science, Tohoku Univerisity, Aoba-ku, Sendai 980-8578, Japan.
Abstract:
In combination with an electrode modified with an abasic site-containing DNA duplex, flavins selectively bind to thymine with a useful electrochemical response, and flavins could be successfully utilized for detection of thymine-related mutations. The abasic site provides hydrophobic microenvironments for the hydrogen bond-forming ligands can recognize the specific target base in the DNA duplex, which is detected by the difference in the electrochemical response.
Related Concept Videos
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Isolation

