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Updated: Jul 18, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Synthesis and application of new amine-modified oligonucleotides using H-phosphonate chemistry
Nagendra Kumar Kamisetty1, Seung Pil Pack, Mitsuru Nonogawa
1Institute of Advanced Energy, Kyoto University, Gokasho, Uji 611-0011, Japan.
Abstract:
Oligodeoxynucleotide probes tethered with amine linkers are commonly used for development of DNA based biosensor tools for studying gene expression and biological assays. However, the current preparation methods for synthesizing amine tethered probes often show defects during acid deprotection and during hydrophobic purification with the amine linker protecting groups. Here, we developed a prospective preparation method of using modified H-phosphonate chemistry to prepare amine tethered oligonucleotide probes. The present developed method will be helpful for preparing efficient amine tethered oligodeoxynucleotide probes that can be used in DNA biosensor applications.
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