Synthesis and properties of DNA oligomers containing 2'-deoxycytidine-N-oxide
Hirosuke Tsunoda1, Akihiro Ohkubo, Haruhiko Taguchi
1Department of Life Science, Tokyo institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama, 226-8501, Japan.
Abstract:
The main product obtained by oxidation of 2'-deoxycytidine with hydrogen peroxide is 2'-deoxycytidine N-oxide. Although its chemical synthesis and properties of 2'-deoxycytidine N-oxide have been well established, little has been reported about the chemical and biochemical behavior of DNA oligomers containing this modified deoxynucleoside. In this study, we synthesized DNA oligomers containing 2'-deoxycytidine N-oxide by use of a post-synthetic oxidative method. It should be noted that this modified base could be incorporated in oligodeoxynucleotides at appropriate positions by this strategy. The ability of the base pair formation was significantly low because of the blocking of the canonical hydrogen bonding site.
Related Concept Videos
Nucleic Acid Structure
DNA Structure
DNA has a double-helix structure. The...
The DNA Helix
The DNA Helix
Nucleic Acids
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic acids
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic Acids and Nucleotides
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria. In...


