Related Experiment Video
Updated: Jul 18, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Published on: July 5, 2024
Functional role of the cofactor on activation process of L-histidine dependent ribozyme
Junji Kawakami1, Chisako Maiya, Naoki Sugimoto
1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Abstract:
The activity of an L-histidine dependent ribozyme was analyzed with many pseudo-cofactors to investigate the activation process of the ribozyme. As a result, only L-histidine was able to induce the ribozyme activity among all chemicals used in this study. On the other hand, many L-histidine analogues inhibited the activation of the ribozyme by L-histidine, indicating the binding site permitted the analogues' access. These results suggest that the ribozyme recognizes L-histidine and express its activity highly specifically via an activation process followed by the histidine binding.
Related Concept Videos
Cofactors and Coenzymes
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Cofactors and Coenzymes
Cofactors and Coenzymes
Ribozymes
Ribozymes can be...
Ribozymes
Ribozymes can be...
tRNA Activation
