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Antisense activity of 2',4'-BNA targeted to bcl-xL gene in HepG2 cell
Somjing Roongjang1, Kurena Takahashi, Myunji Park
1Graduate School of Pharmaceutical Science, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract:
Introduction of the 2',4'-BNA monomer into oligonucleotides significantly enhanced binding affinity toward ssRNA and resistance to nuclease degradation. Here, we evaluated the antisense activity of 2',4'-BNA oligonucleotides against bcl-xL in HepG2 cells. Our data revealed that 2',4'-BNA antisense oligonucleotides remarkably inhibited the expression of bcl-xL in HepG2 cells compared to the natural DNA antisense oligonucleotide. The inhibitory effect of 2',4'-BNA antisense oligonucleotide was dose-dependent and highly sequence-specific.

