Related Experiment Video
Updated: Jul 18, 2026

Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
Published on: May 22, 2012
Estimation of DNA polymerase for improvement of rolling circle amplification
Takeo Yoshimura1, Saori Arikado, Shokichi Ohuchi
1Department of Biological Functions and Engineering, Kyushu Institute of Technology, Wakamatsu, Kitakyushu, Fukuoka 808-0196, Japan.
Abstract:
Rolling Circle Amplification (RCA) is a technique to amplify the oligoncleotides containing tandemly repetitive sequence motif. Various repetitive sequences of which the motif unit is the circular template can be synthesized by extending the primer. Additionally, RCA need not change the temperature regulatory processes like PCR, but RCA has the gene amplification efficiency similar to PCR. Although, it contains the problem of the sequence confirmation and the activity of polymerase as the DNA polymerase I. We comparatively estimated of the various polymerases for the improvement of the RCA. Five DNA polymerases were estimated for RCA.
Related Concept Videos
PCR
PCR - Polymerase Chain Reaction
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Proofreading
Errors During Replication are Corrected by the DNA Polymerase Enzyme
Proofreading
DNA Isolation

