[Separation of bone marrow cells in a Percoll discontinuous density gradient]

Human bone marrow cells were separated by centrifugation in percoll discontinuous density gradient with 1.020-1.080 g/ml densities. CFU-GM test and EPICS analysis were used to characterize cell compositions of the resultant fractions. The employed mode of separation permits obtaining cell fractions rich for monocytes, macrophages, T and B lymphocytes.