Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene trapping in embryonic stem cells.

William L Stanford1, Trevor Epp, Tammy Reid

  • 1Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.

Methods in Enzymology
|December 13, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EpiATLAS - a reference for human epigenomic research.

bioRxiv : the preprint server for biology·2026
Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026
Same author

Enhancing rigor and reproducibility: Implementing STAR Methods and the ISSCR Standards Checklist at Stem Cell Reports.

Stem cell reports·2026
Same author

Stem cell-based embryo models: The 2021 ISSCR stem cell guidelines revisited.

Stem cell reports·2025
Same author

Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma.

Cell death & disease·2025
Same author

Author Correction: A comprehensive human embryo reference tool using single-cell RNA-sequencing data.

Nature methods·2025
Same journal

1,2-Aminothiol-specific conjugation for dual-color fluorescent labeling via ultrafast TAMM conjugates.

Methods in enzymology·2026
Same journal

Nitrone dipoles in bioorthogonal chemistry applications.

Methods in enzymology·2026
Same journal

Bioorthogonal labeling of sialic acid isomers for detection of glycoconjugates by mass spectrometry imaging and microscopy.

Methods in enzymology·2026
Same journal

Bioorthogonal photocatalytic proximity labeling for quantitative mapping of cell-cell interactions.

Methods in enzymology·2026
Same journal

inCu-click: Enabling copper-catalyzed click chemistry inside living cells.

Methods in enzymology·2026
Same journal

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
See all related articles

Gene trapping in embryonic stem cells (ESCs) creates mutations to study gene expression. The International Gene Trap Consortium (IGTC) resource aids researchers in exploring these mutations for gene discovery and functional genomics.

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Gene trapping in embryonic stem cells (ESCs) generates sequence-tagged mutations.
  • This method has been used for gene discovery through expression or function-based screens.
  • The mouse genome sequence has enabled gene trapping to become a high-throughput mutagenesis platform.

Purpose of the Study:

  • To provide a methods guidebook for exploring and utilizing the International Gene Trap Consortium (IGTC) resource.
  • To explain the various vectors and insertions within IGTC libraries.
  • To offer guidance for designing novel expression-based screens.

Main Methods:

  • Utilizing gene trapping in ESCs for mutagenesis.
  • Leveraging the IGTC resource for genome-wide mutation analysis.

Related Experiment Videos

  • Developing and applying expression-based screening strategies.

    • Main Results:

    • The IGTC is creating a comprehensive library of loss-of-function mutations across the mouse genome.
    • Gene trapping now serves as a high-throughput mutagenesis platform.
    • The IGTC resource facilitates exploration of gene function and expression patterns.

    Conclusions:

    • The IGTC resource is a valuable tool for large-scale mutagenesis and gene function studies.
    • Gene trapping remains a powerful strategy for generating and analyzing mutations.
    • Researchers can utilize the IGTC for designing custom genetic screens.