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Published on: May 6, 2016
[The study on culture and trans epithelial electrical resistance of human trabecular cells on filter]
Yehong Zhuo1, Yalin Huang, Yantao Wei
1Key Laboratory of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Purpose:
To establish the culture system of human trabecular cells on filter and detect their TEER (trans epithelial electrical resistance).
Methods:
Immortalized human trabecular cells are cultured on filter. 3.5 days and 1.2 weeks after they were confluent, the TEER were detected to evaluate the resistance of outflow pathway.
Result:
Immortalized human trabecular cells grew well on filter. 3.5 day and 1.2 weeks after their confluence, the average of the TEER were respectively (36.4 +/- 1.4)omega, (35.0 +/- 1.7)omega, (36.1 +/- 2.9)omega, (39.3 +/- 3.0)omega, and the difference between them was not statistically significant (P = 0.305).
Conclusion:
After immortalized human trabecular cells cultured on filter were confluent, their TEER were basically stable at different time. So the culture system of cells on filter and detection of their TEER with EVOM can be used as a good model to study the characteristics of human trabecular cells. Eye
