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Updated: Jul 18, 2026
Oxygen-Induced Retinopathy Model for Ischemic Retinal Diseases in Rodents
Published on: September 16, 2020
[A modified mouse model of oxygen-induced retinopathy]
Xiaoyan Ding1, Xiaoling Liang, Suzhen Xie
1Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Purpose:
To set up a simple and effective oxygen-induced retinopathy (OIR) mouse model.
Methods:
Eyes from P17 mice in modified OIR group(Group A)and traditional OIR group (Group B) were snap frozen for immunohistochemical analysis with antibodies against GSL Flat-mounted, fluorescein-conjugated dextran-perfused retinas were examined to assess the retinal vasculature. The mortality of nursing mothers, survival rate of modeling mice and neovascularization ratio in Group A were obtained and compared with those in Group B.
Results:
Loss of the central retinal vasculature and the neovascular tufts were observed in GSL retinal immunohistochemical analysis. A vascular area and neovasculature area were significantly larger and more typical in Group A than those in Group B. A lower mortality of nursing mothers, higher survival rate and neovascularization ratio of modeling mice was achieved in Group A.
Conclusions:
This modified oxygen-induced retinopathy mouse model is a simple, effective method, with more typical neovasculature were obtained.
