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[The in vitro electrical parameters of the isolated porcine ciliary epithelium]
Yunlan Ling1, Chiho To, Mingkai Lin
1Key Laboratory of Ophthalmology of the Ministry of Education and Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Purpose:
To study the characteristics of ion transport across the isolated porcine ciliary epithelium to offer the good model for human ciliary epithelium study.
Methods:
Fresh isolated pig ciliary epithelium was mounted in a modified Ussing-type chamber to measure the electrical parameters and the effect of 0.1mM ouabain was investigated.
Results:
The potential difference (PD) across the preparations was (-0.6 +/- 0.1) mV and was consistently negative at the aqueous side. The short circuited current (SCC) and resistance (R) were found to be (-10.9 +/- 1.2) microA/cm2 and (54.6 +/- 1.6) omegacm2 respectively. Both PD and SCC were firstly hyperpolarized (stimulation) and then depolarized when 0.1 mM ouabain was applied to the aqueous side.
Conclusion:
The polarity of transepithelial PD of the isolated porcine ciliary epithelium was negative at the aqueous side, implying an anion transport may be important in the aqueous humor formation (AHF). Na+, K+ -ATPase is located on the PE and NPE cells of the porcine ciliary epithelium and plays an important role in
