Fluorescence lifetime correlation spectroscopy
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Updated: Jul 18, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Peter Kapusta1, Michael Wahl, Ales Benda
1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany. photonics@picoquant.com
Abstract:
This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments. The method makes possible to separate the autocorrelation function of individual components of a mixture of fluorophores, as well as purging the result from parasitic contributions like scattered light or detector afterpulsing.
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