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Updated: Jul 18, 2026

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Substructure determination in multiwavelength anomalous diffraction, single anomalous diffraction, and single
G David Smith1, Christopher T Lemke, P Lynne Howell
1Structural Biology and Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Abstract:
A general method for selenium and sulfur substructure determination using the Shake-and-Bake (SnB) algorithm as implemented in SnB in conjunction with anomalous difference E magnitudes is presented. The protocol can be used for Se-Met multiwavelength anomalous diffraction, seleneomethionine single anomalous diffraction (SAD), S-SAD and S/Se-single isomorphous replacement with anomalous scattering data and with minor modifications for other heavy atom derivatives, such as the location of halides.
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