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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
Select bladder smooth muscle cell functions were enhanced on three-dimensional, nano-structured poly(ether urethane)
Megan A Pattison1, Thomas J Webster, Karen M Haberstroh
1Weldon School of Biomedical Engineering, Purdue University 500 Central Drive, West Lafayette, IN 47907-2022, USA.
Journal of Biomaterials Science. Polymer Edition
|December 21, 2006
Summary
Novel nanostructured poly(ether urethane) scaffolds show promise for bladder cancer treatment. These bladder tissue-engineering scaffolds enhance cell adhesion, growth, and extracellular matrix production, outperforming current materials.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Urology
Background:
- Bladder wall resection is a common procedure for invasive bladder cancer, necessitating effective replacement materials.
- Current materials for bladder reconstruction often have limitations in promoting cellular integration and function.
Purpose of the Study:
- To develop and evaluate novel three-dimensional, porous, nanostructured poly(ether urethane) (PU) matrices as scaffolds for bladder tissue engineering.
- To assess the cytocompatibility, extracellular matrix (ECM) production, and mechanical resilience of these nanostructured scaffolds.
Main Methods:
- Fabrication of nanodimensional and microdimensional PU scaffolds.
- In vitro cytocompatibility studies using human bladder smooth muscle cells (BdSMCs), including adhesion and long-term growth assays.
- Quantification of total collagen and elastin in cell-seeded scaffolds.
- Mechanical testing under 10 cmH2O pressure using a computer-controlled pressure chamber.
Main Results:
- Nanodimensional PU scaffolds significantly enhanced BdSMC adhesion, growth, and ECM protein production compared to microdimensional scaffolds.
- Cell-seeded scaffolds maintained their integrity and cellular viability when subjected to 10 cmH2O pressure.
- No adverse effects were observed on scaffolds or cells exposed to pressure compared to controls.
Conclusions:
- The developed nanodimensional PU scaffolds are cytocompatible and support essential cellular functions for bladder tissue regeneration.
- These novel scaffolds demonstrate superior performance over conventional microdimensional scaffolds.
- Nanodimensional PU scaffolds represent a promising biomaterial for bladder tissue engineering and may offer an improved alternative for bladder replacement after resection.
