Detection of hexamethonium-perchlorate association complexes using NACE-MS
1Biotechnology Research Institute, National Research Council of Canada, Montreal PQ, Canada.
Electrophoresis
|December 21, 2006
Summary
This study shows hexamethonium complexes with chlorine oxide anions, enabling detection of perchlorate and other anions in samples using capillary electrophoresis and mass spectrometry.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Chlorine oxide anions, including perchlorate, are environmental contaminants.
- Detection of these anions can be challenging due to their properties.
Purpose of the Study:
- To develop a method for detecting perchlorate and related chlorine oxide anions.
- To investigate the complexation of these anions with hexamethonium.
Main Methods:
- Non-aqueous capillary electrophoresis (NACE) was employed.
- Complexation with hexamethonium (1,6-bis-(trimethylammonium)-hexane) was utilized.
- Mass spectrometry (MS) detection was performed using an Agilent 3DCE system and Bruker Esquire 3000+ mass detector.
Main Results:
- Weak complexation of perchlorate and other chlorine oxide anions with hexamethonium was observed.
- A method detection limit of 10 microg/L for perchlorate in non-aqueous media was achieved.
- Isotope patterns of chlorine (35Cl and 37Cl) enabled unambiguous identification of perchlorate, chlorate, chlorite, and chloride.
Conclusions:
- NACE coupled with MS is effective for identifying chlorine oxide anions.
- The hexamethonium complexation strategy facilitates the analysis of these anions.
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