Related Experiment Video
Updated: Jul 18, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
Application of bovine microsatellite markers for genetic diversity analysis of European bison (Bison bonasus)
T Roth1, I Pfeiffer, K Weising
1Institute of Veterinary Medicine, Georg-August-University of Göttingen, Göttingen, Germany.
Abstract:
In this study, the cross-amplification of a commercial multiplex set of 11 cattle (Bos taurus) microsatellites was tested on a panel of 35 European bison (Bison bonasus) individuals. After polymerase chain reaction optimization, all loci cross-amplified successfully in investigated bisons. Number of alleles and observed and expected heterozygosity per locus are in the range of 2-4, 0.086-0.629 and 0.288-0.621 respectively. The availability of a heterologous set of multiplexed microsatellite markers derived from cattle opens an avenue for collecting profound genetic data for efficient conservation management strategies of the European bison.
Related Concept Videos
Applications of Molecular Taxonomy
Modern Molecular Taxonomy
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

