Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications of Logarithms01:28

Applications of Logarithms

278
Logarithmic functions are powerful tools for simplifying the mathematical representation of phenomena involving exponential changes. Their ability to convert multiplicative relationships into additive ones is especially valuable in various scientific and engineering contexts. One notable application of logarithms is measuring sound intensity, specifically through the decibel (dB) scale used in acoustics.Sound intensity levels vary over an extensive range, from the faintest audible whisper to...
278
Environmental Applications of Microorganisms01:30

Environmental Applications of Microorganisms

1.2K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.2K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K
Radiation: Applications01:17

Radiation: Applications

1.8K
The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...
1.8K
Applications of Stress01:04

Applications of Stress

683
Consider a structure made of a boom and a rod designed to support a load. These two components are connected by a pin and stabilized by brackets and pins. The boom and the rod are detached from their supports to assess the different stresses imposed on this structure, and a free-body diagram is drawn. Then, all the forces applied, including the load acting on the structure, are identified. The reaction forces exerted on both the boom and the rod are computed using the equilibrium equations.
The...
683
Applications of Life Tables01:22

Applications of Life Tables

367
Life tables are versatile across various fields, providing a quantitative basis for analyzing mortality and survival rates. Whether used by demographers, actuaries, epidemiologists, or sociologists, life tables offer valuable insights into the dynamics of life and death, facilitating informed decisions in public health, insurance, conservation, and beyond. Their broad applicability highlights the interconnectedness of demographic data with practical outcomes in everyday life and strategic...
367

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of Pharmacogenetics in Rational Development and Personalization of Drug Treatments.

Personalized medicine·2018
Same author

Integrating genomic-based clinical decision support into electronic health records.

Personalized medicine·2018
Same author

Taxane pharmacogenetics.

Personalized medicine·2018
Same author

Fast and frugal trees: translating population-based pharmacogenomics to medication prioritization.

Personalized medicine·2018
Same author

eHealth: past and future perspectives.

Personalized medicine·2018
Same author

Validation of microRNA pathway polymorphisms in esophageal adenocarcinoma survival.

Cancer medicine·2017
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Pyrosequencing applications.

Sharon Marsh1

  • 1Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 23, 2006
PubMed
Summary
This summary is machine-generated.

Pyrosequencing technology enables rapid genetic analysis, including single-nucleotide polymorphisms and DNA methylation. Advances in multiplexing and universal primers enhance throughput and reduce costs for genetic assays.

More Related Videos

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

48.0K
Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.1K

Related Experiment Videos

Last Updated: Feb 10, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K
Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

48.0K
Pyrosequencing: A Simple Method for Accurate Genotyping
13:06

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

28.1K

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • The human genome sequence has significantly advanced genetics research.
  • New technologies are crucial for high-throughput genetic assays.
  • Pyrosequencing offers a versatile platform for genetic variation analysis.

Purpose of the Study:

  • To review the applications of Pyrosequencing technology in genetic research.
  • To highlight advancements in Pyrosequencing methodology.
  • To demonstrate the utility of Pyrosequencing for various genetic analyses.

Main Methods:

  • Pyrosequencing assays for genetic variation analysis.
  • Application of multiplex and universal primer strategies.
  • Assessment of RNA allelic imbalance, DNA methylation, and gene copy number.

Main Results:

  • Pyrosequencing effectively analyzes single-nucleotide polymorphisms, indels, and short repeats.
  • Methodological improvements have increased assay throughput and reduced costs.
  • The technology is applicable to diverse genetic and epigenetic analyses.

Conclusions:

  • Pyrosequencing is a powerful tool for genetic variation and epigenetic studies.
  • Advancements in Pyrosequencing methodology enhance its efficiency and cost-effectiveness.
  • The technology has broad applications in modern genetics research.