Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplexed proteomic reactor for the processing of proteomic samples.

Weimin Hou1, Martin Ethier, Jeffrey C Smith

  • 1The Ottawa Institute of Systems Biology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, K1H 8M5 Canada.

Analytical Chemistry
|December 30, 2006
PubMed
Summary

We developed a 96-well plate proteomic reactor for efficient, gel-free processing of small protein samples. This new device enables large-scale protein identification from complex biological samples, significantly advancing proteomic analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LC-MS FADE: Leveraging Untargeted Lipidomics Data for Fatty Acid Profiling.

Analytical chemistry·2026
Same author

Functional Synaptic Interactions and Inhibitory Circuitry of the PreBötzinger Complex in the Rhythmic Slice.

bioRxiv : the preprint server for biology·2026
Same author

MetaTree: an interactive web platform for aligned hierarchical data visualization and multi-group comparison.

BMC bioinformatics·2026
Same author

Individual variability shapes ex vivo responses to resistant starch in inflammatory bowel disease derived microbiomes.

NPJ biofilms and microbiomes·2026
Same author

Diving into AA9-mediated transglutaminase 2 inhibition reveals ferroptosis as driver of anticancer effects.

Biochemical pharmacology·2026
Same author

MetaDIA: A DDA-free Database Reduction Strategy for DIA Human Gut Metaproteomics.

Genomics, proteomics & bioinformatics·2026

Area of Science:

  • Proteomics
  • Biochemical Engineering
  • Analytical Chemistry

Background:

  • Minute amounts of complex proteomic samples pose challenges for traditional analysis.
  • Gel-free processing methods are needed for efficient protein analysis.

Purpose of the Study:

  • To develop a novel 96-well plate proteomic reactor for gel-free processing.
  • To enable multiplexed trapping, enrichment, and biochemical processing of proteins.
  • To couple the reactor with size-exclusion chromatography for large-scale protein identification.

Main Methods:

  • Development of a 96-well plate proteomic reactor for gel-free sample processing.
  • Multiplexed trapping, enrichment, and biochemical processing of proteins.
  • Coupling the reactor with size-exclusion chromatography for protein fractionation.

Related Experiment Videos

  • Analysis of MCF7 cell lysate fractions using the reactor and mass spectrometry.

    • Main Results:

    • The reactor can process up to 2 microg of protein per well.
    • Coupling with size-exclusion chromatography enabled large-scale protein identification.
    • Identification of 875 unique proteins under stringent criteria.
    • Identification of 2683 unique proteins at a 1% false positive rate, with 3-10 ng protein lysate per identified protein.

    Conclusions:

    • The 96-well plate proteomic reactor facilitates efficient, gel-free processing of small proteomic samples.
    • This approach enables high-throughput and large-scale protein identification.
    • The developed method significantly enhances proteomic analysis sensitivity and scale.