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Purification of PP2Ac from bovine heart
Hue T Tran1, Tony S Ferrar, Anne Ulke-Lemée
1Department of Biological Sciences, University of Calgary, Alberta, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|January 4, 2007
Abstract:
The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.
