Related Experiment Video
Updated: Jul 17, 2026
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
CYCLONET--an integrated database on cell cycle regulation and carcinogenesis.
Fedor Kolpakov1, Vladimir Poroikov, Ruslan Sharipov
1Institute of Systems Biology, 15, Detskiy proezd, Novosibirsk 630090, Russia.
Nucleic Acids Research
|January 5, 2007
Summary
We developed Cyclonet, a web database integrating omics and chemoinformatics data for mammalian cell cycle regulation. This resource aids anticancer drug discovery by systematically analyzing complex biological information.
Area of Science:
- Computational biology
- Systems biology
- Bioinformatics
Background:
- Mammalian cell cycle regulation is complex, involving numerous interrelated processes.
- Understanding these processes is crucial for developing effective cancer therapies.
- Existing data on cell cycle regulation is often fragmented and difficult to access.
Purpose of the Study:
- To create a comprehensive, web-based database for mammalian cell cycle regulation.
- To integrate diverse data types including 'omics', chemoinformatics, and pathway models.
- To facilitate anticancer drug discovery research by providing a systematic analysis platform.
Main Methods:
- Developed the Cyclonet database, a web-based integrated resource.
- Integrated data from 'omics' sciences and chemoinformatics using a systems biology approach.
- Utilized the BioUML workbench for data querying, analysis, and visual modeling.
Main Results:
- Cyclonet provides access to genes, molecules, regulatory diagrams, simulation results, and microarray data.
- The database includes information on cancer-related gene expression and drug targets.
- It facilitates the prediction of anticancer targets and agents using the Prediction of Activity Spectra for Substances (PASS) method.
Conclusions:
- Cyclonet serves as a specialized resource for researchers in anticancer drug discovery.
- The database enables systematic analysis of cell cycle regulation data.
- It supports the identification of novel anticancer targets and therapeutic strategies.
Related Concept Videos
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
01:28The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...