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Related Experiment Videos

Conditional somatic mutagenesis in the mouse using site-specific recombinases.

R Feil1

  • 1Interfakultäres Institut für Biochemie, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076 Tübingen, Germany. robert.feil@uni-tuebingen.de

Handbook of Experimental Pharmacology
|January 6, 2007
PubMed
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Site-specific recombinases (SSRs) like Cre and Flp enable precise control of gene knockouts in mice. This technology advances the creation of animal models for human diseases and drug discovery.

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Site-specific recombinases (SSRs) are crucial for in vivo genome manipulation.
  • Tools like Cre and Flp allow precise control over gene expression and function in mice.

Purpose of the Study:

  • To review the principles and applications of SSR technology.
  • To focus on conditional somatic mutagenesis and novel applications of SSRs.

Main Methods:

  • Review of Cre/lox system and ligand-activated Cre recombinases.
  • Discussion of strategies for generating and analyzing conditional mouse mutants.
  • Exploration of advanced applications like cell fate mapping.

Main Results:

Related Experiment Videos

  • SSRs provide powerful tools for spatiotemporal control of gene knockouts.
  • Conditional somatic mutagenesis facilitates sophisticated animal model creation.
  • Novel applications expand the utility of SSRs in biological research.

    • Conclusions:

    • SSR technology significantly aids in creating animal models for human disease research.
    • SSRs offer innovative solutions for studying human physiology and pathophysiology.
    • The technology is pivotal for drug discovery and development.