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Updated: Jul 17, 2026
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
Published on: November 27, 2017
Improved embryonic stem cell technologies
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. draper@mshri.on.ca
Handbook of Experimental Pharmacology
|January 6, 2007
Summary
Mouse embryonic stem (ES) cells are vital for studying gene function. New technologies allow precise genetic manipulation for advanced research in vivo and in vitro.
Area of Science:
- Developmental Biology
- Genetics
- Stem Cell Biology
Background:
- Murine embryonic stem (ES) cells are crucial for genetic research.
- Technological advancements have enhanced ES cell capabilities.
Purpose of the Study:
- To review the history and recent progress in mouse ES cell technology.
- To highlight advancements in ES cell derivation and genetic manipulation.
Main Methods:
- Tetraploid aggregation for ES cell manipulation.
- Site-specific recombinases for targeted genetic modification.
- RNA interference (RNAi) for gene function studies.
Main Results:
- Sophisticated manipulation of the ES cell genome is now possible.
- Temporal and spatial control of gene expression is achievable.
- Complex gene function and interaction questions can be addressed.
Conclusions:
- Mouse ES cells are indispensable tools for genetic research.
- Recent technological progress has significantly advanced ES cell applications.
- ES cell-derived chimaeric animals are key for in vivo studies.
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ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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