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[Determination of apoprotein B using the method of solid-phase immunoenzyme analysis]
Indirect solid-phase enzyme immunoassay was used to measure apoB in human blood serum. ApoB obtained by traditional gel filtration in sepharose 4B and high-performance liquid chromatography was used as an antigen. Anti-apoB antibodies were immunochemically identified. Working regimens for the components and conjugates employed were selected. ApoB level in human blood serum, determined by this method, has made up 0.83 +/- 0.1 g/l in men and 0.88 +/- 0.1 g/l in women.
Indirect solid-phase enzyme immunoassay was used to measure apoB in human blood serum. ApoB obtained by traditional gel filtration in sepharose 4B and high-performance liquid chromatography was used as an antigen. Anti-apoB antibodies were immunochemically identified. Working regimens for the components and conjugates employed were selected. ApoB level in human blood serum, determined by this method, has made up 0.83 +/- 0.1 g/l in men and 0.88 +/- 0.1 g/l in women.