[Determination of apoprotein B using the method of solid-phase immunoenzyme analysis].

Area of Science:

• Biochemistry
• Immunology
• Clinical Chemistry

Context:

• Apolipoprotein B (apoB) is a critical component of lipoproteins, playing a vital role in lipid metabolism and cardiovascular health.
• Accurate measurement of serum apoB levels is essential for assessing cardiovascular disease risk.
• Existing methods for apoB quantification may have limitations in sensitivity, specificity, or cost-effectiveness.

Purpose:

• To develop and validate an indirect solid-phase enzyme immunoassay for the quantitative determination of apolipoprotein B (apoB) in human serum.
• To optimize the assay conditions, including antigen preparation, antibody selection, and reagent working regimens.
• To establish reference ranges for serum apoB concentrations in a healthy adult population.

Summary:

• An indirect solid-phase enzyme immunoassay was established using purified apoB (from gel filtration and HPLC) as the antigen and immunochemically identified anti-apoB antibodies.
• Optimal working conditions for all assay components and conjugates were determined.
• The developed immunoassay accurately measured serum apoB levels, yielding values of 0.83 +/- 0.1 g/l in men and 0.88 +/- 0.1 g/l in women.

Impact:

• Provides a reliable and potentially cost-effective method for routine clinical laboratory measurement of serum apoB.
• Facilitates improved cardiovascular risk assessment and patient management through accurate apoB quantification.
• Contributes to a better understanding of apoB reference ranges in the general population.