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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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Published on: November 23, 2010

[An immunoenzyme test system for detecting rickettsiae of the tick-borne spotted fever group]

S N Shpynov, N V Rudakov

    Laboratornoe Delo
    |January 1, 1991
    PubMed

    An enzyme immunoassay (EIA) test system has been developed for the detection of tick spotty fever (TSF) group Rickettsia. A long scheme of immunization in an isogenic system on rabbits has been used with an adjuvant without bacterial components, and based on aluminum hydroxide. This EIA test system detects whole soluble R. akari, R. sibirica, and R. conorii antigens and is at least 10-20 times more sensitive than the indirect hemagglutination test. Treatment in a temperature gradient permits the detection of the corpuscular antigen. A considerable vector infection rate has been revealed in individual examinations of ticks in foci of tick rickettsiasis, whereas such studies carried out in nonendemic areas brought negative results.

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