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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

[Potential and limitations of serological Epstein-Barr virus diagnostics]

D Huzly1, R D Hess

  • 1Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg. daniela.huzly@uniklinik-freiburg.de

Deutsche Medizinische Wochenschrift (1946)
|January 19, 2007
PubMed
Summary

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.