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HAS1 expression in bladder cancer and its relation to urinary HA test
Roozbeh Golshani1, Stefan H Hautmann, Veronica Estrella
1Department of Cell Biology, University of Miami Miller School of Medicine, Miami, Florida 33101, USA.
International Journal of Cancer
|January 19, 2007
Summary
Elevated hyaluronic acid synthase 1 (HAS1) expression in bladder tumors correlates with increased hyaluronic acid (HA) levels and a positive urine HA test, suggesting prognostic potential for bladder cancer.
Area of Science:
- Oncology
- Biochemistry
- Molecular Biology
Background:
- Hyaluronic acid (HA) is elevated in bladder cancer and influences tumor progression.
- Urinary HA levels serve as an accurate diagnostic marker for bladder cancer.
- Hyaluronic acid is synthesized by HA-synthases (HAS1, HAS2, HAS3).
Purpose of the Study:
- To investigate the expression and role of HAS1 in bladder cancer.
- To determine the correlation between HAS1 expression, tissue HA levels, and urinary HA test results.
- To explore the prognostic significance of HAS1 expression in bladder cancer.
Main Methods:
- Real-time RT-PCR and northern blot analysis for HAS1 transcript levels.
- Immunohistochemical analysis for HAS1 and HA protein expression.
- Correlation analysis between HAS1/HA expression and clinical parameters.
Main Results:
- HAS1 transcript levels were significantly elevated (5- to 10-fold) in bladder cancer tissues compared to normal tissues.
- Increased HAS1 expression correlated with higher tissue HA levels and larger HA polymer size.
- HAS1 and HA expression demonstrated high sensitivity and specificity for bladder cancer and correlated with positive urine HA tests.
Conclusions:
- Elevated HAS1 expression in bladder tumors contributes to increased tissue HA and a positive urine HA test.
- HAS1 expression shows potential as a prognostic marker for bladder cancer recurrence and progression.
- HAS1 is a key enzyme in bladder cancer progression and a potential diagnostic/prognostic biomarker.
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