Study of interstitial cells in the penis: human study

Area of Science:

• Urology
• Cell Biology
• Physiology

Background:

• Specialized pacemaker cells, analogous to interstitial cells of Cajal (ICC) in the gut, are known to regulate motility in urinary organs.
• These cells are hypothesized to play a similar role in pacing the motile activity of other organs.

Purpose of the Study:

• To investigate the presence and characteristics of pacemaker cells within the human corpora cavernosa (CC).
• To test the hypothesis that cells similar to ICC exist in the CC and may influence erectile function.

Main Methods:

• Tissue samples (3x3 mm strips) were obtained from the CC of 11 subjects undergoing treatment for Peyronie's disease.
• C-kit immunohistochemistry was performed on the excised CC tissues to identify specific cell populations.
• Controls included incubation with normal rabbit serum to ensure antiserum specificity.

Main Results:

• C-kit positive, branched interstitial cells were identified in the CC.
• These cells were distinct from C-kit negative, non-branched smooth muscle cells.
• While mast cells were also C-kit positive, they exhibited a smooth surface, differentiating them from the target interstitial cells.

Conclusions:

• Interstitial cells resembling ICC have been identified in the human CC.
• These cells are likely responsible for initiating slow waves and controlling smooth muscle activity within the CC.
• A potential deficiency or absence of these cells may negatively impact erectile function, warranting further research into their role in erection.