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Detection of dopamine receptor subtypes in the rat diaphragm
Janet D Pierce1, Jay Jegathesan, Beth Levant
1School of Nursing, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
Background:
The administration of dopamine using an in vivo animal model has been shown to prevent and treat diaphragm fatigue.
Objective:
With the knowledge that dopamine stimulates alpha- and beta-adrenergic and dopamine receptors, the purpose of this study was to investigate whether dopamine receptors are present in the diaphragm. In addition, if dopamine receptors were detected, we would identify the class and subtype of dopamine receptors.
Method:
Radioligand binding studies and reverse transcriptase polymerase chain reaction experiments were used.
Results:
The receptor binding studies were unable to establish whether dopamine receptors were present in the diaphragm. However, highly sensitive reverse transcriptase polymerase chain reaction experiments demonstrated that D(1)-like receptors (D(1) and D(5) receptors) were detected in the diaphragm.
Conclusions:
This study is the first to report the class and specific subtype of dopamine receptors found in the diaphragm. By identifying dopamine receptors in the diaphragm, we have a better understanding of the mechanisms by which dopamine treats and prevents diaphragm fatigue.
