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Updated: Jul 17, 2026
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Large Scale Zebrafish-Based In vivo Small Molecule Screen
Published on: December 30, 2010
Small-molecule regulation of zebrafish gene expression
Hanife Esengil1, Vicky Chang, John K Mich
1Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, Center for Clinical Sciences Research, Room 3155, Stanford, California 94305, USA.
Nature Chemical Biology
|January 24, 2007
Summary
Scientists developed a new chemical tool for zebrafish research. This system allows precise control over gene expression, advancing the study of human diseases in this model organism.
Area of Science:
- * Zebrafish as a model organism in biomedical research.
- * Development of novel genetic tools for reverse genetics.
Background:
- * Limited availability of conditional reverse-genetic tools in zebrafish hinders research.
- * Need for precise gene expression control in model organisms.
Purpose of the Study:
- * To introduce a novel chemically inducible gene expression technology for zebrafish.
- * To enable multidimensional control of gene expression in zebrafish models.
Main Methods:
- * Development of a chemically inducible gene expression system.
- * Demonstration of orthogonality to vertebrate signaling pathways.
- * Utilizing tissue-specific promoters for targeted gene induction.
Main Results:
- * Achieved high induction levels and rapid kinetics of gene expression.
- * System shows orthogonality to endogenous vertebrate signaling.
- * Enabled precise, multidimensional control over gene expression.
Conclusions:
- * The new technology overcomes limitations in zebrafish reverse genetics.
- * Facilitates the creation of advanced zebrafish models for human diseases.
- * Enhances the utility of zebrafish in biomedical research.
