Related Experiment Video
Updated: Jul 17, 2026
08:20
Using Microarrays to Interrogate Microenvironmental Impact on Cellular Phenotypes in Cancer
Published on: May 21, 2019
A perspective on microarrays: current applications, pitfalls, and potential uses
Pratik Jaluria1, Konstantinos Konstantopoulos, Michael Betenbaugh
1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 221 Maryland Hall, 3400 North Charles Street, Baltimore, MD 21218, USA. pratikj@mail.nih.gov
Microbial Cell Factories
|January 27, 2007
Summary
Microarray technology offers versatile applications across various scientific fields, from diagnostics to forensics. Careful experimental design and data interpretation are crucial for extracting valuable insights from this powerful genomic tool.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Microarray technology has advanced significantly due to improvements in robotics, computation, and high-quality slide fabrication.
- Its applications span diverse fields including toxicology, evolutionary biology, drug development, diagnostics, and forensics.
- Many researchers lack foundational knowledge of microarray principles and experimental design, hindering effective data utilization.
Purpose of the Study:
- To outline key features of microarray technology for researchers unfamiliar with its principles and applications.
- To provide guidance on experimental design, statistical methods, and interpretation of microarray data.
- To highlight the versatility and future potential of microarray technology.
Main Methods:
- Review of fundamental principles of microarray technology.
- Analysis of current applications based on recent publications.
- Discussion of essential considerations for experimental design and data analysis.
Main Results:
- Microarray technology is a powerful tool with broad applicability in scientific research.
- Effective use requires careful planning, understanding of limitations, and appropriate statistical methods.
- The review highlights key concepts for conducting and interpreting microarray experiments.
Conclusions:
- Microarray technology is a versatile tool with significant potential across multiple scientific disciplines.
- Successful implementation relies on a thorough understanding of its principles and careful experimental design.
- This review provides a substantive overview to aid researchers in leveraging microarray data effectively.
Related Concept Videos
02:34DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...