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Updated: Jul 17, 2026

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy
Published on: June 13, 2013
[Expression of mACh receptor ml-m5 subunits in the vestibular endorgans of rat]
Huanmao Cheng1, Weijia Kong, Qi Yao
1Department of Otolaryngology, the First People's Hospital, Jingmen, 438000, China.
Objective:
To investigate the expression of mACh receptor ml - m5 subunits in the vestibular endorgans of rat.
Method:
Gene specific primers used were based upon the rat mACh receptor ml-m5 subunits sequence. Reverse transcription polymerase chain reaction (RT-PCR) was carried out on the vestibular endorgans. The PCR products were directly sequenced.
Result:
It was shown that mACh receptor ml - m5 subunits mRNA was detected and sequenced from vestibular endorgans by RT-PCR technique. Sequence analysis of the PCR products confirmed identity to rat mACh receptor ml--m5 subunits cDNA sequence in the predicted region.
Conclusion:
Present data confirmed the expression of ACh receptor ml-m5 subunits in the vestibular endorgans of rat, giving the direct evidence to date that ACh is one of the important neurotransmitters in the peripheral vestibular system of rat.
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