Multichannel recording and stimulation of neuronal cultures grown on microstamped poly-D-lysine

We report progress toward designable, reproducible, patterned in vitro neuronal cultures. Cell adhesive proteins were directly microcontact printed on microelectrode arrays (MEAs) used as substrates for hippocampal neurons grown in a defined culture medium. The patterned neuronal network circuits were maintained up to one month. The recorded activity was comparable to that of cultures grown on unpatterned uniform surfaces. Time-locked evoked responses were recorded across the networks.