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Updated: Jul 17, 2026
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Published on: May 7, 2012
[Specific cytotoxicity of CTL which was induced by DC pulsed with mannosylated antigen]
Guo-cheng Zhong1, Ming Xu, Ming Shi
1Department of Oncology, 452 Hospital, Chengdu 610021, China. zgc005@yahoo.com.cn
Aim:
To investigate the role of mannosylated tumor antigen in the course of anti-tumor response.
Methods:
L2 domain of HER-2/neu (ErbB2) ectodomain was expressed in E.coli, purified and mannosylated. Dendritic cells(DCs) were induced from human peripheral blood mononuclear cell by GM-CSF and IL-4. The maturation and functional capacity of DCs pulsed with mannosylated L2 (mL2) protein was investigated.
Results:
L2 protein could induce DC maturation, which was accompanied by elevated expression of MHC and costimulatory molecules. The effect of mL2 protein on DC maturation was more remarkable than that of non-mannosylated L2 protein. Furthermore, DCs pulsed with mL2 could stimulate high tumor cell lysis by CTL.
Conclusion:
Our experiment provide the foundation for the study of new tumor vaccine by mannosylation.
